Table 1.
List of primers used in real-time quantitative PCR
| Primer ID | Sequence |
|---|---|
| GAPDH F | 5’-GAAGGTGAAGGTCGGAGTC-3’ |
| GAPDH R | 5’-GAAGATGGTGATGGGATTTC-3’ |
| CD19 F | 5’-TGCCCCGTCTTATGGAAACC-3’ |
| CD19 R | 5’-CTCTTCTTCTGGGCCCACTC-3’ |
| AXIN 2 F | 5’-ACTGCCCACACGATAAGGAG-3’ |
| AXIN 2 R | 5’-CTGGCTATGTCTTTGCACCA-3’ |
| TCF1 F | 5’-TGCAGCTATACCCAGGCTGG-3’ |
| TCF1 R | 5’-CCTCGACCGCCTCTTCTTC-3’ |
| MYC F | 5’-TACCCTCTCAACGACAGCAG-3’ |
| MYC R | 5’-TCTTGACATTCTCCTCGGTG-3’ |
| WNT1 F | 5’-CGGCGTTTATCTTCGCTATC-3’ |
| WNT1 R | 5’-TTCGATGGAACCTTCTGAGC-3’ |
| WNT2 F | 5’-AAAGAAGATGGGAAGCGCCA-3’ |
| WNT2 R | 5’-TTCATCAGGGCTCTGGCATC-3’ |
| WNT3A F | 5’-TGTTGGGCCACAGTATTCCT-3’ |
| WNT3A R | 5’-GGGCATGATCTCCACGTAGT-3’ |
| WNT6 F | 5’-CAGCCCCTTGGTTATGGAC-3’ |
| WNT6 R | 5’-AACTGGAACTGGCACTCTCG-3’ |
| WNT8A F | 5’-AGGCTGAGAAGTGCTACCAGA-3’ |
| WNT8A R | 5’-CCATTGTTTGACCCATCACA-3’ |
| FZD1 F | 5’-CATCGTCATCGCCTGCTACT-3’ |
| FZD1 R | 5’-TAGCGTAGCTCTTGCAGCTC-3’ |
| FZD2 F | 5’-CTTCTCACAGGAGGAGACGC-3’ |
| FZD2 R | 5’-AAATGATAGGCCGCTCTGGG-3’ |
| FZD3 F | 5’-ACAGCAAAGTGAGCAGCTACC-3’ |
| FZD3 R | 5’-CTGTAACTGCAGGGCGTGTA-3’ |
| FZD4 F | 5’-TCAAGAGACGCTGTGAACCC-3’ |
| FZD4 R | 5’-GGTCGTTCTGTGGTGGGAAT-3’ |
| FZD5 F | 5’-GCACAACCACATCCACTACG-3’ |
| FZD5 R | 5’-GCACAACCACATCCACTACG-3’ |
| FZD6 F | 5’-AGGCTTGCACCGTTTTGTTC-3’ |
| FZD6 R | 5’-TGCTCGATGGCTTCACAACT-3’ |
| FZD7 F | 5’-CGCCTCTGTTCGTCTACCTC-3’ |
| FZD7 R | 5’-TCATGATGGTGCGGATACGG-3’ |
| FZD8 F | 5’-CGCCTCTGTTCGTCTACCTC-3’ |
| FZD8 R | 5’-TCATGATGGTGCGGATACGG-3’ |
| FZD9 F | 5’-TGCTCACCTTCTTGCTGGAG-3’ |
| FZD9 R | 5’-GCCAGCGAGTAGACGTTGTA-3’ |
| FZD10 F | 5’-AAGAAGAGCCGGAGAAAACC-3’ |
| FZD10 R | 5’-GACTGGGCAGGGATCTCATA-3’ |
| LRP5 F | 5’-CACCACCTTCTTGCTGTTCA-3’ |
| LRP5 R | 5’-GCTTTGACGTTCCTCAGTCC-3’ |
| LRP6 F | 5’-GACTGGGTTGCACGAAATCT-3’ |
| LRP6 R | 5’-CGGGGTTCCTCTAAGTCCTC-3’ |