Fig. 4 |. PAH-1 contributes to serotonin-related behaviors.

a, BAS-1∷SL2∷GFP∷H2B is expressed in neuronal cells, as well as in the epidermis and intestine. Scale bar for all images, 15 μm. At least ten animals were scored at day 1 of adulthood for GFP expression under well fed conditions on at least two different days. b, Loss of PAH-1 significantly increases exploration behavior after 13.5 h to a similar extent as loss of TPH-1. c, 1 mM exogenous NAS partially rescued increased exploration behavior of bas-1 mutants after 13.5 h. d, Total number of eggs laid in response to SSRIs fluoxetine (0.5 mg ml⁻¹) and imipramine (0.75 mg ml⁻¹) were determined within 2 h of drug exposure in 1-day old (d) and 2-day old (e) WT (N2; black), tph-1(mg280) (green), pah-1(syb3601) (blue) and pah-1(syb3596); tph-1(mg280) (red) hermaphrodites. Data in b (n = 20 per experiment per genotype and treatment) were collected in two biologically independent experiments. Data in c (n = 10 and n = 11 for the first two experiments and n = 12 for the third experiment), data in d (n = 8) and e (n = 8) were collected in three biologically independent experiments. Statistical significance (b–e) was determined using one-way ANOVA with Tukey’s post hoc test.