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. Author manuscript; available in PMC: 2024 Feb 1.
Published in final edited form as: Nat Chem Biol. 2022 Oct 10;19(2):141–150. doi: 10.1038/s41589-022-01148-7

Extended Data Fig. 4 |. Serotonin-related behaviors of different mutants.

Extended Data Fig. 4 |

a, Heat map of locomotory behaviors of pah-1(syb3601), tph-1(mg280), pah-1(syb3596);tph-1(mg280) compared to WT. Fed day-1 adult animals were tracked using an automated single worm tracker. pah-1(syb3601) mutant animals (n = 46) did not show any gross defects in locomotion compared to WT. Features significantly different (q-value(Wilcoxon) <0.05) between WT (n = 42) and tph-1(mg280) (n = 40) mutant animals are displayed as fold change in relation to WT (red: upregulated compared to WT; blue: downregulated compared to WT). Except for foraging, pah-1(syb3596);tph-1(mg280) double mutants (n = 47) display significant changes in the same locomotory features compared to WT as observed between WT and tph-1(mg280) animals. n.s., not significant. b, Loss of PAH-1 did not result in gross locomotory defects. Fed day 1 adult animals were tracked using an automated single worm tracker. pah-1(syb3601) mutant animals did not show any gross defects in locomotion compared to WT. Features significantly different (q-value (Wilcoxon) <0.05) between WT and tph-1(mg280) mutant animals are displayed as boxplots with individual values for each animal tested. Additional loss of PAH-1 does not exacerbate phenotypes observed in tph-1(mg280) single mutants. Boxplots were generated using Worm Tracker (v2.0). Red center line indicates mean with 95 % confidence intervals (white box), outer boxes indicate standard deviation. c, Loss of PAH-1 significantly increased exploratory behavior after 6 h, to a larger extent than loss of TPH-1. d, Exogenous NAS did not influence exploratory behavior of WT after 13.5 h. Data in c (n = 20 per experiment per genotype and treatment) were collected in 2 biologically independent experiments. Data in d (n = 10 and n = 11 for the first two experiments and n = 12 for the third experiment) were collected in 3 biologically independent. Statistics in b-d were calculated using One-Way-ANOVA and p-values were adjusted with posthoc Tukey’s multiple comparison test. n.s., not significant.