Table 2:
Electrophysiological properties of GlyT2-GFP+ interneurons in WT and SOD1 animals.
| Parameters | Groups | Statistics | ||
|---|---|---|---|---|
| WT (Mean ± SD) | SOD (Mean ± SD) | p | g (95% CI low, high) | |
| N = 34 | N = 25 | |||
| Resting membrane potential (mV) | −46.7 ± 6.4 | −45.7 ± 6.9 | 0.524K | 0.155 (−0.385, 0.705) |
| Capacitance (pF) | 66 ± 42 | 55 ± 27 | 0.529K | −0.289 (−0.725, 0.210) |
| Input resistance (MΩ) | 205 ± 104 | 220 ± 131 | 0.794K | 0.127 (−0.398, 0.657) |
| PIC properties | ||||
| PIC onset (mV) | −50.5 ± 6.0 | −46.2 ± 5.0 | 0.004 | 0.765 (0.254, 1.217) |
| PIC peak (mV) | −35.2 ± 4.9 | −31.2 ± 4.6 | 0.003 | 0.820 (0.299, 1.285) |
| PIC amplitude (pA) | 100 ± 73 | 97 ± 62 | 0.890K | −0.037 (−0.520, 0.494) |
| Normalized PIC (pA/pF) | 1.79 ± 1.4 | 1.92 ± 1.1 | 0.226K | 0.100 (−0.354, 0.666) |
| Firing properties | ||||
| Inst firing freq range max (Hz) | 112 ± 44 | 113 ± 42 | 0.961 | 0.013 (−0.493, 0.557) |
| Steady state firing freq range min (Hz) | 6 ± 2.4 | 6 ± 3.2 | 0.453K | 0.277 (−0.282, 0.831) |
| I-ON (pA) | −51 ± 111 | −14 ± 53 | 0.093K | 0.392 (−0.113, 0.672) |
| I-OFF (pA) | −5 ± 66 | 36 ± 69 | 0.038K | 0.286 (−0.324, 0.852) |
| Δ I (pA) | 47 ± 59 | 50 ± 52 | 0.530K | −0.140 (−0.555, 0.453) |
| AP properties | ||||
| AP overshoot past 0 (mV) | 19.9 ± 9.8 | 21.4 ± 7.9 | 0.529 | 0.156 (−0.346, 0.670) |
| AP duration at half peak (ms) | 1.54 ± 0.54 | 1.48 ± 0.40 | 0.944K | −0.118 (−0.559, 0.425) |
| AP rate of rise (V/s) | 87 ± 29 | 83 ± 13 | 0.446K | 0.172 (−0.616, 0.328) |
| AP rate of fall (V/s) | 45 ± 17 | 43 ± 11 | 0.995K | 0.113 (−0.398, 0.593) |
| Threshold on ramp (mV) | −40.6 ± 4.4 | −37.9 ± 5.7 | 0.076K | 0.542 (−0.011, 1.050) |
| Threshold on step (mV) | −47.3 ± 4.4 | −45.1 ± 6.2 | 0.199K | 0.413 (−0.191, 0.879) |
| AHP properties | ||||
| AHP amplitude (mV) | 11.2 ± 3.4 | 11.0 ± 3.9 | 0.704K | −0.047 (−0.565, 0.480) |
| AHP duration (s) | 0.37 ± 0.21 | 0.29 ± 0.19 | 0.207K | −0.353 (−0.867, 0.171) |
| AHP duration at half amplitude (s) | 0.12 ± 0.06 | 0.10 ± 0.06 | 0.045K | −0.370 (−0.873, 0.221) |
| AHP tau (s−1) | 0.117 ± 0.115 | 0.105 ± 0.083 | 0.980K | −0.121 (−0.552, 0.426) |
| IH properties | ||||
| Hyperpolarizing Sag (%) | 32 ± 18 | 32 ± 21 | 0.537K | −0.011 (−0.563, 0.556) |
| Depolarizing Rebound (%) | 17 ± 15 | 19 ± 24 | 0.536K | 0.086 (−0.454, 0.698) |
N is the number of cells included in analysis.
K
Indicates Kruskal-Wallis Test for nonparametric distributions; all other p values were calculated using ANOVA analysis.
Values of g are determined using Hedge’s statistic to calculate effect size and include the 95% confidence interval of the effect size