Table 4:
Electrophysiological properties of WT and SOD1 GlyT2-GFP+ interneurons in lamina IX.
| WT (Mean ± SD) | SOD (Mean ± SD) | p | g (95% CI low, high) | |
|---|---|---|---|---|
| N = 14 | N = 11 | |||
| PIC Onset (mV) | −50.5 (± 6.7) | −44.0 (± 5.0) | 0.013 | 1.046 (0.340, 1.652) |
| PIC Peak (mV) | −33.1 (± 3.9) | −31.1 (± 4.5) | 0.248 | 0.462 (−0.349, 1.165) |
| PIC Amplitude (pA) | 116 (± 81) | 98 (± 76) | 0.412K | −0.218 (−0.944, 0.655) |
| I-ON (pA) | −69 (± 143) | −11 (± 64) | 0.155K | 0.485 (−0.439, 0.928) |
| I-OFF (pA) | −23 (± 72) | 22 (± 73) | 0.080K | 0.602 (−0.401, 1.207) |
| Δ I (pA) | 48 (± 76) | 33 (± 30) | 0.702K | −0.226 (−0.564, 1.021) |
| Threshold on ramp (mV) | −40.7 (± 4.9) | −37.8 (± 6.8) | 0.244 | 0.473 (−0.370, 1.209) |
| Threshold on step (mV) | −47.4 (± 4.2) | −43.9 (± 7.4) | 0.112K | 0.595 (−0.437, 1.377) |
| AHP amplitude (mV) | 11.4 (± 3.9) | 10.2 (± 3.5) | 0.421 | −0.319 (−1.09, 0.514) |
| AHP duration (s) | 0.41 (± 0.24) | 0.25 (± 0.16) | 0.062 | −0.764 (−1.509, 0.053) |
| AHP tau (s−1) | 0.129 (± 0.118) | 0.076 (± 0.067) | 0.274K | −0.511 (−1.088, 0.390) |
| Morphology of Patched interneurons in lamina IX | ||||
| N = 14 | N = 10 | |||
| Soma | ||||
| Soma Surface Area (μm2) | 703 (± 242) | 734 (± 284) | 0.771 | 0.118 (−0.740, 0.959) |
| Soma Volume (μm3) | 1732 (± 1080) | 1989 (± 1149) | 0.412K | 0.224 (−0.620, 1.076) |
| Primary Neurites | ||||
| Total Neurite Length (μm) | 101 (± 130) | 129 (± 60) | 0.046K | 0.258 (−0.610, 1.855) |
| Average Neurite Length (μm) | 29 (± 24) | 37 (± 16) | 0.069 K | 0.335 (−0.544, 1.442) |
| Total Neurite Surface Area (μm2) | 545 (± 524) | 721 (± 293) | 0.089K | 0.384 (−0.522, 1.722) |
| Average Neurite Surface Area (μm2) | 164 (± 99) | 208 (± 80) | 0.128 K | 0.466 (−0435, 1.381) |
| Total Neurite Volume (μm3) | 309 (± 283) | 388 (± 161) | 0.114K | 0.316 (−0.498, 1.474) |
| Average Neurite Volume (μm3) | 95 (± 65) | 113 (± 46) | 0.178 K | 0.307 (−0.496, 1.233) |
| Morphology of Unpatched interneurons in lamina IX | ||||
| N = 310 | N = 258 | |||
| Soma Surface Area (μm2) | 459 (± 164) | 372 (± 169) | 0.069LM | |
| Soma Volume (μm3) | 525 (± 257) | 392 (± 248) | 0.067LM | |
K
Indicates Kruskal-Wallis Test for nonparametric distributions
LM
Indicates linear mixed effects model; all others were ANOVA analysis.
Values of g are determined using Hedge’s statistic to calculate effect size and include the 95% confidence interval of the effect size