Table 5:
Electrophysiological properties of WT and SOD1 GlyT2-GFP+ interneurons in Intermediate Region of lamina VII and VIII.
| Parameters | Groups | |||
|---|---|---|---|---|
| WT (Mean ± SD) | SOD (Mean ± SD) | p | g (95% CI low, high) | |
| N = 16 | N = 8 | |||
| PIC Onset (mV) | −49 (± 5) | −47 (± 4) | 0.435 | 0.332 (−0.451, 1.240) |
| PIC Peak (mV) | −36 (± 4) | −32 (± 5) | 0.046 | 0.884 (−0.210, 1.796) |
| PIC Amplitude (pA) | 92 (± 67) | 77 (± 50) | 0.881K | −0.235 (−0.914, 0.667) |
| I-ON (pA) | −18 (± 45) | −16 (± 54) | 0.776K | 0.035 (−1.219, 0.762) |
| I-OFF (pA) | 21 (± 47) | 74 (± 76) | 0.101K | 0.877 (−0.038, 1.904) |
| Δ I (pA) | 39 (± 35) | 90 (± 69) | 0.076K | 1.006 (−0.077, 2.145) |
| Threshold on ramp (mV) | −38.9 (± 3.1) | −37.0 (± 6.3) | 0.525K | 0.439 (−0.701, 1.495) |
| Threshold on step (mV) | −46.7 (± 4.7) | −46.3 (± 6.4) | 0.490K | 0.080 (−0.850, 1.319) |
| AHP amplitude (mV) | 11.3 (± 3.2) | 10.5 (± 5.3) | 0.658 | −0.198 (−1.375, 0.853) |
| AHP duration (s) | 0.34 (± 0.21) | 0.25 (± 0.23) | 0.341K | −0.401 (−1.189, 0.843) |
| AHP tau (s−1) | 0.119 (± 0.127) | 0.127 (± 0.125) | 1.000K | 0.062 (−0.657, 1.381) |
| Morphology of Patched interneurons in laminae VII and VIII. | ||||
| N = 15 | N = 8 | |||
| Soma | ||||
| Soma Surface Area (μm2) | 574 (± 244) | 559 (± 160) | 0.699K | −0.066 (−0.733, 0.847) |
| Soma Volume (μm3) | 1232 (± 919) | 1228 (± 570) | 0.776K | 0.005 (−0.645, 0.956) |
| Primary Neurites | ||||
| Total Neurite Length (μm) | 110 (± 80) | 77 (± 33) | 0.220K | −0.466 (−0.888, 0.435) |
| Average Neurite Length (μm) | 30 (± 18) | 24 (± 8) | 0.606 K | −0.352 (−0.813, 0.700) |
| Total Neurite Surface Area (μm2) | 506 (± 321) | 514 (± 265) | 0.699K | 0.026 (−0.665, 0.994) |
| Average Neurite Surface Area (μm2) | 139 (± 72) | 168 (± 85) | 0.366 K | 0.365 (−0.419, 1.393) |
| Total Neurite Volume (μm3) | 234 (± 189) | 330 (± 210) | 0.272K | 0.474 (−0.394, 1.45) |
| Average Neurite Volume (μm3) | 64 (± 46) | 109 (± 70) | 0.071 K | 0.782 (−0.150, 1.730) |
| Morphology of Unpatched interneurons in laminae VII and VIII. | ||||
| N = 655 | N = 812 | |||
| Soma Surface Area (μm2) | 448 (± 225) | 394 (± 178) | 0.495LM | |
| Soma Volume (μm3) | 510 (± 329) | 425 (± 253) | 0.455 LM | |
K
Indicates Kruskal-Wallis Test for nonparametric distributions
LM
Indicates linear mixed effects model; all others were ANOVA analysis.
Values of g are determined using Hedge’s statistic to calculate effect size and include the 95% confidence interval of the effect size