Figure 2.

Detection of FG4R via OmpG^L3-FLAG nanopore. a) Schematic model of OmpG^L3-FLAG sensor and FG4R depicted to relative scale. b,c,d) Representative current traces of OmpG^L3-FLAG in the b) absence or c-d) presence of FG4R (20 nM). Regions of the trace showing FG4R binding are colored in orange. The red and blue dotted lines represent I₀ and FG4R-bound current respectively. e) All-points histogram of 30 second segments of OmpG^L3-FLAG during I₀ and FG4R bound states. Experiments were performed in 50 mM Na₂HPO₄ pH 6.0 buffer containing 300 mM KCl at ±50 mV. Traces were filtered using a 500 Hz lowpass digital gaussian filter.