Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-CGRP | Immunostar | Cat#24112 |
| Griffonia anti-IB4 | Life Tech | Cat#I21411 |
| Chicken anti-GFP | Life Tech | Cat#A10262 |
| Rabbit anti-RFP | Rockland | Cat#600401379 |
| Rabbit anti-HA-Tag | Cell Signaling | Cat#3724 |
| Bacterial and virus strains | ||
| pAAV.CAG.LSL.tdTomato | Addgene | Plasmid#100048 |
| AAV-EF1a-N-CretrcintG | Addgene | Plasmid#69571 |
| AAV-EF1a-C-CreintG | Addgene | Plasmid#69570 |
| AAV1-syn-FLEX-jGCaMP8m | Addgene | Plasmid#162378-AAV1 |
| pENN.AAV.CB7.Cl.mCerulean.WPRE.RBG | Addgene | Plasmid#105557-AAV9 |
| pAAV-hSyn-GRAB_DA2m | Addgene | Plasmid#140553 |
| pAAV-hSyn-GRAB_DA1h | Addgene | Plasmid#113050 |
| pAAV-Ef1a-DIO-ChRmine-mScarlet-3xHA (PHP.S) | Dr. David Ng | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Bicuculline | Alomone | Cat #: B-137 |
| Strychnine | Sigma | Cat #: S8753-25G |
| CNQX | Alomone | Cat #: C-141 |
| TTX | Caymen Chemical | Cat #: 14964 |
| Estradiol benzoate | Sigma | Cat#E1600000 |
| Progesterone | Sigma | Cat#p0130-100g |
| Tamoxifen | Sigma | Cat#T5648-1G |
| Critical commercial assays | ||
| RNAScope Probe: eYFP | ACD | Cat#312131 |
| RNAScope Probe: Mrgprb4 | ACD | Cat#435781 |
| RNAScope Probe: Mrgprd | ACD | Cat#417921 |
| RNAScope Probe: Mrgprc11 | ACD | Cat#488771 |
| RNAScope Probe: Mrgpra3 | ACD | Cat#548161 |
| RNAScope Probe: Pou4f2 | ACD | Cat#558481 |
| RNAScope Probe: Trpa1 | ACD | Cat#400211 |
| RNAScope Probe: Ret | ACD | Cat#431791 |
| RNAScope Probe: Th | ACD | Cat#317621 |
| RNAScope Probe: Scn10a | ACD | Cat#426011 |
| RNAScope Probe: Calca | ACD | Cat#578771 |
| RNAScope Probe: Gfra1 | ACD | Cat#431781 |
| RNAScope Probe: Trpv1 | ACD | Cat#313331 |
| RNAScope Intro Pack for Multiplex Fluorescence | ACD | Cat#323136 |
| RNAScope Multiplex Fluorescent Reagent Kit v2 | ACD | Cat#323100 |
| Opal 520 Reagent Pack | Perkin Elmer | FP1487001KT |
| Opal 570 Reagent Pack | Perkin Elmer | FP1488001KT |
| Opal 690 Reagent Pack | Perkin Elmer | FP1497001KT |
| Experimental models: Organisms/strains | ||
| Mouse: Mrgprb4tdTomato-Cre | Jackson Laboratories | Strain #: 021077 |
| Mouse: Rosa26Ai32-eYFP | Jackson Laboratories | Strain #: 012569 |
| Mouse: Tg(Gpr83-EGFP)DV46Gsat/Mmucd | MMRRC, UC Davis | Stock #: 010442-UCD |
| Mouse: Rosa26DTA | Jackson Laboratories | Strain #: 009669 |
| Mouse: DATIREScre | Jackson Laboratories | Strain #: 006660 |
| Mouse: MrgprdCreERT2 | Jackson Laboratories | Strain #: 031286 |
| Mouse: Mrgpra3Cre-EGFP | Dr. Xinzong Dong | N/A |
| Software and algorithms | ||
| Synapse software | TDT | N/A |
| Clampex software | Molecular Devices | N/A |
| PAWS software | Jones et al.37 | N/A |
| ProAnalyst | Xcitex | N/A |
| pMAT software | Bruno et al.71 | https://github.com/djamesbarker/pMAT |
| CNMF-E | Zhou et al.70 | N/A |
| Inscopix Data Processing Software | Inscopix | Version 1.6.0 |
| Inscopix Data Acquisition Software | Inscopix | Version 1.3.0 |
| Other | ||
| Zeiss Axiocam 506 color camera | Zeiss | N/A |
| MultiClamp 700B amplifier | Molecular Devices | N/A |
| Axon Digidata 1550B | Molecular Devices | N/A |
| Siskiyou MX7600 Motorized Micromanipulator | Siskiyou | MX7600 |
| Conditioned Place Preference Chamber | Rossi et al.68 | N/A |
| FastCAM UX100 high speed camera | Photron | 800K-M-4GB |
| 473 nm blue laser light | Shanghai Laser and Optics, China | BL473T8-150FC/ADR-800A |
| 532 nm green laser | Shanghai Laser and Optics Century, Shanghai, China | GL532T8-1000FC/ADR-800A |
| 593 nm continuous wave diode pumped solid-state orange laser | CrystalLaser LC | CL593-050-O |
| 10MHZ Function Waveform Generator | Agilent | 33210A |
| FC/PC Optogenetic patch cable | ThorLabs | M72L01 |
| TDT Fiber Photometry RZ10X Real Time Processor | TDT | RZ10X |
| TDT Experiment Control Workstation | TDT | WS-4 |
| Mono Fiber-optic Cannula | Doric | MFC_200/230-0.57_6mm_MF1.25_FLT |
| Super glue | Loctite | LOC1255800 |
| Fiber Photometry Fluorescence Mini Cubes – 6 ports | Doric | FMC6_IE(400-410)_E1(460-490)_F1(500-540)_E2(555-570)_F2(580-680)_S |
| Zirconia mating sleeves | Doric | SLEEVE_ZR_1.25_BK |
| Mono Fiber-optic Patch Cords | Doric | MFP_200/230/900-0.57_1m_FCM-MF1.25(F)_LAF |
| Metabond Quick Adhesive Cement System | Parkell | SKU: S380 |
| Ortho-Jet Liquid Dental cement | Lang Dental | N/A |
| GRIN lens 7.3mm length, NA 0.5 | Inscopix | 1050-004413 |
| Laser power meter | ThorLabs | s120c |
| Syringe pump | Harvard Apparatus | PHD Ultra |