Table 3.

Manufacturer-provided equations to calculate metabolic endpoints, i.e. basal respiration, glycolysis, from each assay trace.

Assay	Metabolic Endpoint	Equation
Metabolic Phenotype	Metabolic potential	$\left(\frac{\text{Stressed OCR or ECAR}}{\text{Baseline OCR or ECAR}}\right)\times 100$
Cell Mito Stress	Non- mitochondrial oxygen consumption	Minimum rate measurement after rotenone and antimycin injection
	Basal respiration	(Last rate measurement before first injection) – (Non-mitochondrial respiration)
	Maximal respiration	(Maximum rate measurement after oligomycin injection) – (Non-mitochondrial respiration)
	ATP production	(Last rate measurement before oligomycin injection) – (Minimum rate measurement after oligomycin injection)
	Proton leak	(Minimum rate measurement after oligomycin injection) – (Non-mitochondrial respiration)
	Coupling efficiency	$\left(\frac{\text{ATP Production Rate}}{\text{Basal Respiration Rate}}\right)\times 100$
Glycolysis Stress	Glycolysis	(Maximum rate measurement before oligomycin injection) – (Last rate measurement before glucose)
	Glycolytic capacity	(Maximum rate measurement after oligomycin injection) – (Last rate measurement before glucose injection)
	Glycolytic reserve	(Glycolytic Capacity) – (Glycolysis)
	Non-Glycolytic acidification	Last rate measurement prior to glucose injection