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. 2023 Feb 3;14(2):80. doi: 10.1038/s41419-023-05628-z

Fig. 4. TMEM105 enhances breast cancer cell invasion and liver metastasis via promoting LDHA expression.

Fig. 4

Wound healing assay was performed to detect the effects of LDHA knockdown or overexpression on the migration of TMEM105-overexpressing MCF-7 cells (A) and TMEM105-knockdown MDA-MB-231 cells (B). Transwell matrigel assay was performed to detect the effects of LDHA knockdown or overexpression on the invasion of TMEM105-overexpressing MCF-7 cells (C) and TMEM105-knockdown MDA-MB-231 cells (D). E Representative HE images of the livers from nude mice without or with BCLM. Scale bar, 50 μm. F, G The BCLM in the mice was determined by qRT-PCR assays. H Western blot analysis of LDHA expression in liver metastatic lesions from nude mice injected with engineered breast cancer cells. I, J Changes of the overall survival in nude mice injected with engineered MCF-7 (I) and MDA-MB-231 (J) cells (n = 8 for each group). **p < 0.01.