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. 2023 Feb 3;14(2):80. doi: 10.1038/s41419-023-05628-z

Fig. 8. Lactate promotes TMEM105 expression by SHH-MAZ signaling pathway.

Fig. 8

A The lactate secretion inhibitor DCA (2 mM) downregulated TMEM105 expression in MCF-7 and MDA-MB-231 cells treated with lactate (10 mM) for 24 h. B TMEM105 expression was determined by qRT-PCR in MCF-7 and MDA-MB-231 cells treated with different concentrations of lactate for 24 h. C The schematic images of the MAZ binding motif and the potential binding sites of MAZ in the TMEM105 promoter. D ChIP assay was used to detect the roles of MAZ binding sites on the TMEM105 promoter region. E TMEM105 reporter vectors with truncated mutants in the promoter region at site P1, site P2, or both sites were transfected into MCF-7 or MDA-MB-231 cells treated with lactate (10 mM), and luciferase activity was detected. F SHH and MAZ expression in adjacent normal and breast cancer tissues in the TCGA dataset. G Expression of SHH, SMO, and GLI1 was examined in breast cancer cells using Western blot. H Effects of cyclopamine (10 μM) on TMEM105 expression in breast cancer cells treated with lactate (10 mM). **p < 0.01.