Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 6;55(1):120–131. doi: 10.1038/s12276-022-00909-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Scatterplots of Cdon expression in aortic samples from patients with atherosclerotic plaques (GSE43292, n = 32) and calcified aortas (GSE12644 and GSE83453, n = 22). Statistical significance was determined with a two-tailed Student’s t-test. **P < 0.01. b Uniform manifold approximation and projection (UMAP) visualization of VSMCs (upper box: all genes in VSMCs, bottom box: only Cdon expression in VSMCs) in calcified atherosclerotic core plaques (AC) and patient-matched proximal adjacent portions (PA) of the carotid artery (GSE159677, n = 3). c Cdon expression in PA VSMCs and AC VSMCs (n = 3). Statistical significance was determined with a two-tailed Student’s t-test. ***P < 0.005. d Representative immunostaining images of Cdon and αSMA in aortas injected with VD3. Scale bar: 100 μm (top) and 50 μm (bottom). e Quantification of the intensity of Cdon fluorescence normalized to the intensity of αSMA, as shown in Panel d (n = 4). Data represent the means ± SEMs analyzed by Student’s t-test. *P < 0.05. f Relative RNA expression level of Cdon in aortic samples from VD3-injected mice (n = 3). Data represent the means ± SEMs analyzed by Student’s t-test. **P < 0.01. g Immunoblot analysis of VSMCs cultured with CM. h The relative RNA expression of Cdon in CM-treated VSMCs. Data represent the means ± SEMs analyzed by Student’s t-test. *P < 0.05.