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. 2023 Jan 6;55(1):120–131. doi: 10.1038/s12276-022-00909-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Relative Axin2 transcript levels in the vehicle- and VD3-treated aortas (n = 3). Data represent the means ± SEMs analyzed by one-way ANOVA. *P < 0.05, **P < 0.01. b Relative Axin2 transcript levels in the control or Cdon-deficient cells (n = 3). Data represent the means ± SEMs analyzed by Student’s t-test. *P < 0.05. c Immunoblot analysis of the shCont- or shCdon lentivirus-infected VSMCs. d Top-flash reporter activity of the control or Cdon-depleted VSMCs in response to Wnt3a (n = 3). Data represent the means ± SEMs analyzed by one-way ANOVA. *P < 0.05, **P < 0.01. e Immunoblot analysis of the shCont- or shCdon-infected VSMCs in response to DMSO or XAV939. f Relative Axin2 expression in control- or Cdon-overexpressing VSMCs treated with Wnt3a (n = 3). Data represent the means ± SEMs analyzed by one-way ANOVA. **P < 0.01, ***P < 0.005. g The TopFlash reporter activity of the control- or Cdon-overexpressing VSMCs in response to Wnt3a (n = 3). Data represent the means ± SEMs analyzed by Student’s t-test and one-way ANOVA. ***P < 0.005. h Immunoblot analysis of the control- or Cdon-overexpressing VSMCs in response to Wnt3a.