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. 2023 Jan 6;55(1):120–131. doi: 10.1038/s12276-022-00909-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Immunoblot analysis of Cdon-Fc (the entire Cdon ectodomain fused to Fc) and Ig2-Fc (Ig2 domain fused to Fc) purified from cell supernatants. b Immunoblot analysis of VSMCs treated with CM and each purified protein. IgG served as a control. c Alizarin Red staining images of the VSMCs treated with control or CM in combination with Cdon-Fc, Ig2-Fc, or IgG. Scale bar = 100 μm. d Quantification of Alizarin Red staining shown in panel (c) (n = 3). Data represent the means ± SEMs analyzed by one-way ANOVA. ***P < 0.005. e Relative transcript levels of Axin2, Runx2, and ALPL in the VSMCs treated with control or CM in combination with Cdon-Fc, Ig2-Fc, or IgG.