Preparation of peritoneal wall and omentum samples for WMI and confocal microscopy studies
(A) Description of the aluminum weight used for WMI and confocal microscopy imaging of peritoneal wall samples.
(B) Description of the aluminum weight used for WMI and confocal microscopy imaging of omentum samples.
(C) Surgical tool set used for this protocol including (from left to right) straight blunt 11 cm scissors, straight sharp 8.5 cm scissors, 45° angled serrated 16 cm forceps, standard straight serrated 13 cm forceps and fine straight serrated 13 cm forceps.
(D) Incision in the skin in the abdominal area.
(E and F) Dissection of the peritoneal wall.
(G) Peritoneal wall flattened and stretched on the bottom of the 60-mm Petri dish.
(H–J) Dissection of the omentum. (I) Magnification of the area marked by a white lined square in (H).