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. 2023 Jan 4;55(1):95–107. doi: 10.1038/s12276-022-00916-8

Fig. 6. ED_miR-3144(3_A < G) functions as an onco-miR in liver cancer.

Fig. 6

a Cell growth was measured by MTT assay after transfection. b Cells were transfected with the ED_miR-3144(3_A < G) mimic or cotransfected with the mimic and antisense ED_miR-3144(3_A < G) (AS-ED_miR-3144(3_A < G)) (upper). miR-3144-3p or the ED_miR-3144(3_A < G) mimic was ectopically transfected into cells (middle). Western blot (middle) and luciferase reporter assays (lower). c Western blotting was performed after transfection of primary mir-3144 or cotransfection of miR-3144 with siADAR1 into Hep3B and Huh7 cells (upper). A luciferase reporter assay was performed with MIHA and SNU-449 cells (lower). d Western blot analysis showing the direct regulatory effect of ED_miR-3144(3_A < G) on SLC38A4 expression. e Cells were cotransfected with wild-type SLC38A4 or a pcDNA3.1_SLC38A4 mutant with primary mir-3144. All data are shown as the mean ± SEM; *P < 0.05, **P < 0.01 by unpaired Student’s t-test.