Fig. 6.

CCL22 is responsible for the pro-EMT effect of LINC00662-induced macrophages. A A wound healing assay was performed using LINC00662-transfected or NC-transfected THP-1-treated OS cells. OS cells were co-cultured with M0 or M2 macrophages. B Transwell assay was performed using the OS cells co-cultured with the indicated macrophages. Scale bar, 100 μm. C Western blot analyses of EMT biomarker expression were performed using OS cells co-cultured with the indicated macrophages. D Relative expression of cytokine mRNA expression levels in LINC00662-transfected macrophages versus control macrophages was detected by qRT-PCR. E Secreted CCL22 levels in M0, M2, control or LINC00662-transfected macrophages conditioned media determined by ELISA. F Transwell assay was used to measure the migration of CCL22-treated OS cells, and OS cells co-cultured with M0 or M2 macrophages. Scale bar, 100 μm. G A wound healing assay was used to measure the migration of CCL22-treated OS cells, and OS cells co-cultured with M0 or M2 macrophages. H Western blot analyses of EMT biomarker expression in the indicated cells. I Transwell assays were used to measure the migration of OS cells co-cultured with control or LINC00662-transfected macrophages with or without anti-CCL22 antibody. Scale bar, 100 μm. J A wound healing assay was used to determine the migration of OS cells co-cultured with control or LINC00662-transfected macrophages with or without anti-CCL22 antibody. K Western blot analyses of EMT biomarker expression in OS cells co-cultured with control or LINC00662-transfected macrophages with or without anti-CCL22 antibody. L Schematic diagram of the project