Fig. 1.

Inhibition of biofilm formation a of S. aureus ATCC 25922 (MSSA) and b of S. aureus ST80 (MRSA), by SPT. Bacteria were grown under stative conditions, in the presence or absence of several concentrations of STP (0.03–20 μg/mL) in 96-well TCPs. Bars represent mean values ± SEM from at least three independent experiments, with the value of the untreated bacterial culture (control) set at 100%. Brown-Forsythe tests and Welch ANOVA were employed for the statistical analysis. Notations for statistically significant differences between control (untreated) and treated samples: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. c Biofilms formed on glass slides in the absence (−SPT) or presence (+SPT) of SPT and stained with CV. Images are taken from × 100 magnification under a light microscope