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Cell electrospinning (CE) promotes a higher degree of maturation of stem cell-derived smooth muscle cells (SMCs) than cell printing (CP). (a) α-SMA/DAPI staining on day 21 and quantification of the (b) alpha smooth muscle actin (α-SMA) aspect ratio and (c) α-SMA orientation factor. (d) Calponin/DAPI staining on day 21 and (e) analysis of relative smooth muscle-specific gene expression. GAPDH was used to normalize gene expression. Data indicate mean ± SD (n = 3).
