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. 2023 Jan 11;102(4):102473. doi: 10.1016/j.psj.2022.102473

Figure 2.

Figure 2

Alpha diversity (Shannon's index) box plots show (a) significant difference between ileal and cecal microbiota (T-test, P > 0.001), (b) no significant effect of treatments on ileal microbiota diversity (ANOVA, P = 0.180), (c) no significant effect of treatment on ceca microbiota (ANOVA, P = 0.320). Treatments include— A) Negative Control treatment- chicks fed a basal corn-soybean meal-wheat–based diet; (B) In-feed antibiotics- chicks fed NC + 0.05% bacitracin methylene disalicylate; (C) In-feed probiotics- chicks fed NC + 0.005% Bacillus subtilis containing 1 × 108 CFU/kg of feed; (E) In ovo probiotics group 1- eggs injected with 10 × 106 CFU of Bacillus subtilis fermentation extract /0.2 mL saline diluent; and (F) In ovo probiotics group 2- eggs injected with 20 × 106 CFU of Bacillus subtilis fermentation extract /0.2 mL saline diluent. Boxes in the boxplots denote interquartile range, solid middle line in the boxes denote the median, and dotted lines denote the means, all symbols outsides the boxes represent outliers.