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. 2023 Jan 11;102(4):102473. doi: 10.1016/j.psj.2022.102473

Table 1.

Ingredients, calculated, and analyzed compositions of experimental diets1 (as-fed basis, percentage, unless otherwise stated).

Ingredients Phases
Starter (0–14 d)
Grower (15–25 d)
Finisher (26–35 d)
Negative Control In-feed Antibiotic In-feed probiotic Negative Control In-feed Antibiotic In-feed probiotic Negative Control In-feed Antibiotic In-feed probiotic
Ingredient composition
Corn (ground) 46.63 46.53 46.62 51.16 51.06 51.15 53.63 53.53 53.62
Soybean meal-46.5 37.12 37.14 37.13 31.87 31.89 31.88 29.2 29.22 29.21
Wheat 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0
Soybean Oil (young or mature) 1.80 1.83 1.795 2.18 2.21 2.175 2.75 2.78 2.75
Limestone 1.37 1.37 1.37 1.30 1.30 1.30 1.19 1.19 1.19
Dicalcium Phosphate 21 P 1.45 1.45 1.45 1.35 1.35 1.35 1.18 1.18 1.18
DL Methionine premix2 0.58 0.58 0.58 0.57 0.57 0.57 0.52 0.52 0.52
Vitamin/Mineral Premix or MCB10 3,4 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50
Salt 0.38 0.38 0.38 0.36 0.36 0.36 0.36 0.36 0.36
Lysine HCl 0.17 0.17 0.17 0.21 0.21 0.21 0.17 0.17 0.17
Pellet Binding Agent - - - 0.50 0.50 0.50 0.50 0.50 0.50
BMD 110G5 - 0.05 - - 0.05 - - 0.05 -
Bacillus subtilis - - 0.005 - 0.005 - - 0.005
Total 100 100 100 100 100 100 100 100 100
Nutrient Calculated composition
Metabolizable energy (kcal/kg) 2,975 2,975 2,975 3,025 3,025 3,025 3,100 3,100 3,100
Crude protein 22.0 22.0 22.0 20.0 20 20.0 19.0 19.0 19.0
Calcium 0.90 0.90 0.90 0.84 0.84 0.84 0.76 0.76 0.76
Available phosphorus 0.45 0.45 0.45 0.42 0.42 0.42 0.38 0.38 0.38
Sodium 0.18 0.18 0.18 0.17 0.17 0.17 0.17 0.17 0.17
Digestible lysine 1.22 1.22 1.22 1.12 1.12 1.12 1.02 1.02 1.02
Digestible methionine + cysteine 0.91 0.91 0.91 0.85 0.85 0.85 0.80 0.80 0.80
Digestible Tryptophan 0.24 0.24 0.24 0.22 0.22 0.22 0.20 0.20 0.20
Digestible Threonine 0.84 0.84 0.84 0.76 0.76 0.76 0.72 0.72 0.72
Analyzed composition
Dry Matter 92.2 92.2 92.2 91.5 92.1 91.4 91.7 91.8 91.8
Crude protein 24.5 24.7 23.9 21.3 21.2 21.8 19.3 20.9 21.0
Crude fat 4.05 4.31 4.17 4.86 4.69 3.63 4.81 4.25 4.17
Calcium 0.81 0.80 1.03 0.89 0.90 0.83 0.83 0.75 0.75
Potassium 1.05 1.00 0.98 0.94 0.91 0.95 0.84 0.92 0.89
Phosphorus 0.62 0.65 0.72 0.66 0.65 0.65 0.57 0.60 0.59
Sodium 0.14 0.15 0.20 0.17 0.17 0.17 0.16 0.15 0.15
1

Basal diet (NC); In-feed antibiotic diet containing NC + 0.05% bacitracin methylene disalicylate (BMD); In-feed probiotics diet containing NC + 0.005% Bacillus subtilis containing- 1 × 108 CFU/kg of feed.

2

Supplied/kg premix: DL-Methionine, 0.5 kg; wheat middling, 0.5 kg.

3

Starter vitamin-mineral premix contained the following per kg of diet: 9750 IU vitamin A; 2000 IU vitamin D3; 25 IU vitamin E; 2.97 mg vitamin K; 7.6 mg riboflavin; 13.5 mg Dl Ca-pantothenate; 0.012 mg vitamin B12; 29.7 mg niacin; 1.0 mg folic acid, 801 mg choline; 0.3 mg biotin; 4.9 mg pyridoxine; 2.9 mg thiamine; 70.2 mg manganese; 80.0 mg zinc; 25 mg copper; 0.15 mg selenium; 50 mg ethoxyquin; 1543 mg wheat middling's; 500 mg ground limestone.

4

Grower and Finisher vitamin-mineral premix contained the following per kg of diet: 9750 IU vitamin A; 2000 IU vitamin D3; 25 IU vitamin E; 2.97 mg vitamin K; 7.6 mg riboflavin; 13.5 mg Dl Ca-pantothenate; 0.012 mg vitamin B12; 29.7 mg niacin; 1.0 mg folic acid, 801 mg choline; 0.3 mg biotin; 4.9 mg pyridoxine; 2.9 mg thiamine; 70.2 mg manganese; 80.0 mg zinc; 25 mg copper; 0.15 mg selenium; 50 mg ethoxyquin; 1543 mg wheat middling's; 500 mg ground limestone.

5

Bacitracin methylene disalicylate (providing 55 mg/kg mixed feed); Alpharma, Inc., Fort Lee, NJ, USA.