Table 2.
Effect of in ovo delivery of Bacillus subtilis on hatch performance and chick quality.
| Hatch parameters | Noninjected | Treatments1 |
SEM2 | P value3 | ||
|---|---|---|---|---|---|---|
| In ovo saline | In ovo probiotic 1 | In ovo probiotic 2 | ||||
| Hatchability (%) | 96.1 | 95.2 | 96.8 | 96.9 | 0.51 | 0.711 |
| Average chick weight (g) | 43.1 | 43.8 | 43.4 | 43.7 | 0.13 | 0.118 |
| Average chick length (cm) | 18.8 | 18.2 | 19.2 | 18.9 | 0.18 | 0.202 |
| Chick BW/ egg weight (%) | 68.1b | 69.5a | 68.9ab | 69.2a | 0.17 | 0.005 |
| Average navel score | 1.40 | 1.38 | 1.47 | 1.36 | 0.07 | 0.79 |
1
Treatments include— (1) noninjected eggs; (2) in ovo saline group- injected with 0.2 mL of physiological saline (0.9% NaCl); (3) in ovo probiotics group 1- eggs injected with 10 × 106 CFU of Bacillus subtilis fermentation extract/0.2 mL saline diluent; and (4) in ovo probiotics group 2- eggs injected with 20 × 106 CFU of Bacillus subtilis fermentation extract/0.2 mL saline diluent.
2
SEM = Standard error of means.
3
Means within a row with different superscripts.
a,b
significantly differ.