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. 2023 Jan 11;102(4):102473. doi: 10.1016/j.psj.2022.102473

Table 3.

Effect of Bacillus subtilis and its delivery routes on the growth performance of broiler chickens raised for 35 days.

Growth performance parameters Treatments1
 P value3
Negative control In-feed antibiotic In-feed probiotic In ovo probiotic 1 In ovo probiotic 2 SEM2
Week 1
Average feed intake (g) 155 153 159 160 154 1.52 0.859
Average body weight gain (g) 85.7 87.4 91.6 88.7 74.1 1.00 0.071
FCR4 1.81 1.76 1.73 1.81 2.07 0.02 0.336
Week 2
Average feed intake (g) 184 190 148 163 168 3.94 0.237
Average body weight gain (g) 213 235 212 200 168 3.09 0.102
FCR4 0.87 0.81 0.69 0.81 0.99 0.02 0.193
Week 3
Average feed intake (g) 449 499 416 466 409 13.4 0.552
Average body weight gain (g) 483 555 480 476 441 9.07 0.188
FCR4 0.93 0.89 0.87 0.99 0.93 0.03 0.912
Week 4
Average feed intake (g) 839 917 812 859 853 16.6 0.222
Average body weight gain (g)-Mixed sex 714 608 695 773 846 15.2 0.415
Average body weight gain (g)-Males 761 722 902 954 918 17.7 0.351
Average body weight gain (g)-Females 635 483 524 677 820 21.9 0.293
FCR4 1.31 1.24 1.43 1.38 1.39 0.09 0.516
Week 5
Average feed intake (g) 1,378ab 1,329a 1,146b 1,102ab 970b 14.8 0.024
Average body weight gain (g)-Mixed sex 1,030 1,181 906 789 710 143 0.324
Average body weight gain (g)-Males 970 1157 936 926 857 504 0.850
Average body weight gain (g)-Females 977 1,019 831 679 625 42.1 0.412
FCR4 1.37 1.14 1.27 1.43 1.48 0.04 0.655
Total Trial Period (1-35 d)
Average feed intake (g) 2,974 3,051 2,656 2,753 2,595 34.4 0.087
Average body weight gain (g) 2,578 2,655 2,385 2,353 2,217 139 0.574
FCR4 1.16 1.16 1.13 1.20 1.20 0.02 0.830
1

Treatments include— (1) Negative Control treatment- chicks fed a basal corn-soybean meal-wheat–based diet; (2) In-feed antibiotics- chicks fed NC + 0.05% bacitracin methylene disalicylate; (3) In-feed probiotics- chicks fed NC + 0.005% Bacillus subtilis containing 1 × 108 CFU/kg of feed; (4) In ovo probiotics group 1- eggs injected with 10 × 106 CFU of Bacillus subtilis fermentation extract /0.2 mL saline diluent; and (5) In ovo probiotics group 2- eggs injected with 20 × 106 CFU of Bacillus subtilis fermentation extract /0.2 mL saline diluent.

2

SEM = Standard error of means.

3

Means within a row with different superscripts.

a,b

Significantly differ.

4

FCR = Feed Conversion Ratio.