Table 1.
Cuticular hydrocarbon biosynthesis candidate genes in the mason wasp Odynerus spinipes.
| Wasp gene ID | DGE between | Honey bee gene ID | %ID | Predicted function | Cell type |
|---|---|---|---|---|---|
| g3158 | age classes | GB52590 | 54.7 | fatty acid synthase | oenocytes (weak) |
| g6537 | age classes | GB46038 | 85.9 | fatty acid elongase | oenocytes + trophocytes |
| g7609 | age classes | GB54399 | 58.7 | fatty acid elongase | oenocytes (weak) |
| g7610 | age classes | GB54397 | 55.2 | fatty acid elongase | oenocytes |
| g7616 | age classes / chemotypes | GB51247 | 82.9 | fatty acid elongase | oenocytes |
| g7620 | age classes | GB54401 | 84.0 | fatty acid elongase | trophocytes |
| g7621 | age classes | GB54302 | 67.7 | fatty acid elongase | NA |
| g14708 | age classes | NAa | NAa | fatty acid desaturase | oenocytes |
| g14712 | chemotypes | GB42218 | 54.2 | fatty acid desaturase | oenocytes |
| GB51236 | 64.1 | oenocytes | |||
| GB51238 | 55.2 | trophocytes | |||
| g2413 | age classes | GB50627 | 64.2 | fatty acyl-CoA reductase | oenocytes |
| g1571 | age classes | GB52087 | 48.1 | fatty acyl-CoA reductase | oenocytes + trophocytes |
| g7842 | age classes | GB49380 | 72.3 | fatty acyl-CoA reductase | oenocytes |
| g283 | chemotypes | GB44756 | 36.9 | rhythmically expressed gene 5 protein | NA |
| g2290 | chemotypes | GB53695b | 34.3 | fatty acid amide hydrolase | oenocytes + trophocytes (weak) |
| g3059 | chemotypes | GB52820 | 62.4 | parathyroid hormone-related peptide receptor-like protein | oenocytes (weak) + trophocytes (weak) + hexagonal cells (strong) |
The table informs about 1) the mason wasp gene ID, 2) between which groups differential expression differences (DGE) were detected, 3) orthologous/homologous genes in the honey bee (Apis mellifera) genome, 4) the percentage of identical amino acids between the encoded sequences of the mason wasp and those of the honey bee, 5) the genes’ predicted functions, and 6) the expression of the candidate genes in honey bee fat body cells.
aSince we found no 1:1 ortholog in the honey bee genome, we assessed the phylogenetically most closely related homolog, GB40659 (39.6%), for being involved in CHC biosynthesis.
bOne of three co-orthologs in the honey bee, the other two being GB48850 (36.4%) and GB47832 (34.3%) (not studied by us).