Table 3.
PCR-RFLP genotyping of T. gondii isolates from cat-hunted wild small mammals.
| Rodent ID | Host species | Group/Location | Tissue | PCR-RFLP allele markers |
RFLP-ToxoDB genotype | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SAG1 | 3′-SAG2 | 5′-SAG2 | Alt.SAG2 | SAG3 | BTUB | GRA6 | C22-8 | C29-2 | L358 | PK1 | Apico | |||||
| 36 | Arvicola amphibius | Group 1/Finstersee, Zug | Brain | II/III | II | I/II | II | II | II | II | II | II | II | x | I | x |
| 55 | Myodes glareolus | Group 1/Finstersee, Zug | Brain | II/III | II | I/II | II | II | II | II | II | II | II | II | I | #3 |
| 4 | Apodemus flavicollis | Group 1/Finstersee, Zug | Brain | II/III | II | I/II | II | x | II | I | x | x | II | II | I | x |
| B42 | Arvicola amphibius | Group 3/Oey, Bern | Brain | II/III | II | I/II | II | II | II | II | II | II | II | II | I | #3 |
| B44 | Arvicola amphibius | Group 3/Oey, Bern | Masseter muscle | II/III | II | I/II | II | II | II | II | II | II | II | II | I | #3 |
Note: GenBank accession numbers of the marker sequences obtained in this study: 3′SAG2:OQ102531-102535; Apico: OQ102536-102540; BTUB: OQ102541-102545; c22-8:OQ102546-102549; c29-2: OQ102550-102553; L358: OQ102554-102558
x: not amplified