Table 2.
Central composite design (CCD) matrix for amylase derived from selected thermophilic bacteria.
| Experimental variables |
Response |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Runs | Temperature | Incubation periods | Inocula volumes | pH | Substrate con. | Unit activity (MBT001) | Unit activity (MBT002) | Unit activity (MBT003) | Unit activity (MBT004) |
| 1 | 70 | 48 | 100 | 5 | 1.5 | 2.32496 | 2.00785 | 2.20044 | 2.23785 |
| 2 | 70 | 48 | 100 | 6 | 1.5 | 2.89402 | 2.8832 | 2.53142 | 2.71325 |
| 3 | 70 | 48 | 100 | 7 | 1.5 | 3.23041 | 3.01973 | 3.01156 | 3.20973 |
| 4 | 70 | 48 | 100 | 8 | 1.5 | 3.6209 | 4.00355 | 4.00013 | 4.0135 |
| 5 | 70 | 48 | 100 | 9 | 1.5 | 2.25239 | 2.16921 | 2.316 | 2.20692 |
| 6 | 70 | 48 | 100 | 7 | 0.5 | 2.57429 | 2.70734 | 2.5593 | 2.51584 |
| 7 | 70 | 48 | 100 | 7 | 1 | 2.75164 | 2.77329 | 2.59344 | 2.68003 |
| 8 | 70 | 48 | 100 | 7 | 1.5 | 3.05555 | 3.15797 | 3.0414 | 2.99913 |
| 9 | 70 | 48 | 100 | 7 | 2 | 2.17087 | 2.51251 | 2.35655 | 2.48228 |
| 10 | 70 | 48 | 100 | 7 | 1.5 | 3.05555 | 3.15797 | 3.0414 | 2.56513 |
| 11 | 70 | 48 | 200 | 7 | 1.5 | 3.06804 | 3.15963 | 3.08886 | 3.04473 |
| 12 | 70 | 48 | 300 | 7 | 1.5 | 3.08719 | 3.1155 | 3.15797 | 3.17629 |
| 13 | 70 | 48 | 400 | 7 | 1.5 | 4.10884 | 4.1 2525 | 3.29119 | 3.90818 |
| 14 | 70 | 48 | 500 | 7 | 1.5 | 3.95536 | 3.95289 | 4.62508 | 4.54182 |
| 15 | 70 | 24 | 100 | 7 | 1.5 | 2.55264 | 2.8757 | 2.6792 | 2.54515 |
| 16 | 70 | 48 | 100 | 7 | 1.5 | 3.05555 | 3.15797 | 3.0414 | 2.99913 |
| 17 | 70 | 72 | 100 | 7 | 1.5 | 2.21613 | 2.20704 | 2.00459 | 2.05703 |
| 18 | 60 | 48 | 100 | 7 | 1.5 | 2.35031 | 3.03141 | 2.58012 | 2.54681 |
| 19 | 70 | 48 | 100 | 7 | 1.5 | 3.05555 | 3.15797 | 3.0414 | 2.85129 |
| 20 | 80 | 48 | 100 | 7 | 1.5 | 2.04723 | 2.3578 | 2.52097 | 2.21517 |