Table 3.
Group differences in the methylation levels of the SLC6A4 promoter region.
| CpG units | All AN(n = 91) | AN-R(n = 44) | AN-BP(n = 47) | HC(n = 87) | AN vs. HC t/Z (P) | AN-R vs. AN-BP vs. HC F/H (P) |
|---|---|---|---|---|---|---|
| CpG1 | 9.32 ± 3.16 | 10.14 ± 3.03 | 8.55 ± 3.14 | 9.03 ± 3.60 | 0.960 (0.337) | 5.141 (0.076) |
| CpG3 | 4.29 ± 2.54 | 4.12 ± 2.62 | 4.41 ± 2.51 | 4.99 ± 2.62 | −1.754 (0.081) | 1.743 (0.178) |
| CpG26.27.28 | 5.08 ± 2.45 | 4.86 ± 2.16 | 5.80 ± 3.81 | 4.32 ± 2.57 | 2.065 (0.039) | 4.810 (0.090) |
| CpG31.32 | 2.52 ± 2.99 | 1.81 ± 2.19 | 3.08 ± 3.46 | 1.71 ± 2.42 | 2.038 (0.042)a | 6.676 (0.036)a |
| CpG33.34.35.36 | 5.51 ± 2.08 | 5.17 ± 2.00 | 5.76 ± 2.16 | 5.21 ± 1.61 | 1.113 (0.266) | 2.004 (0.367) |
| CpG37 | 6.51 ± 2.94 | 6.69 ± 2.66 | 6.29 ± 3.17 | 5.57 ± 2.92 | 2.358 (0.018) | 5.175 (0.075) |
| CpG39.40 | 4.68 ± 2.28 | 4.20 ± 2.00 | 4.94 ± 2.44 | 4.26 ± 2.20 | 1.255 (0.210) | 2.854 (0.240) |
AN: anorexia nervosa; AN-R: anorexia nervosa restricting subtype; AN-BP: anorexia nervosa binge/purging subtype; HC: healthy control.
a
Methylation level of the CpG31.32 was non-normally distributed, the Mann-whitney U test and Kruskal-Wallis H test were used; methylation levels of other CpG units were normally distributed, T test and ANOVA test were used.