Fig. 2.

Selection of lncRNAs for the FIMICS panel. The flowchart represents the workflow and displays the numbers of lncRNAs at each step of the selection process. From left to right: the grey boxes represent the RNA-seq datasets used for the analysis. The green box shows known lncRNAs detected from GENCODE19 (10,482), the light blue box shows novel transcripts identified (13,664), the Venn diagram in pink box shows the known lncRNAs selected by different strategies [differentially expressed (DE) in HF (green), highly expressed (yellow) and cardiac enriched (blue)], the Venn diagram in yellow box shows the novel lncRNAs [DE in HF (green), cardiac enriched (blue), and removed due to high correlation to known genes (grey striped circle)]. 2211 known and 1036 novel lncRNAs were identified (orange boxes), of which 3233 (dark blue box) were included in the FIMICS panel after removal of 14 lncRNAs due to sequence redundancy. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)