Table 2.
Summary of the sedimentation experiments and modeling for 10 collagen peptides
| Collagen peptides | AUC (light water) |
AUC (heavy water) |
Theoretical s20, w (S) |
|
|---|---|---|---|---|
| s20, w (S) | s20, w (S) | Linear model(hydrated) | Best-fit MD model | |
| MBL-C | 1.20 ± 0.01 | 1.42 ± 0.01 | 0.88 | 0.96 |
| MBL-N | 1.15 ± 0.06 | 1.31 ± 0.03 | 0.89 | 0.97 |
| MBL-E | 1.09 ± 0.04 | 1.51 ± 0.10 | 0.93 | 1.02 |
| MBL-12 | 1.30 ± 0.03 | 1.55 ± 0.02 | 1.00 | 1.10 |
| (POG)10 | 1.28 ± 0.10 | 1.39 ± 0.10 | 0.88 | 0.96 |
| (POG)13 | 1.30 ± 0.04 | 1.59 ± 0.09 | 0.97 | 1.11 |
| (POG)14 | 1.61 ± 0.10 | n.a. | 1.00 | n.a. |
| (PPG)10 | 0.95 ± 0.03 | n.a. | 0.90 | 0.97 |
| G>A | 1.19 ± 0.10 | n.a. | 0.88 | 0.96 |
| T3-785 | 0.95 ± 0.08 | n.a. | 0.87 | 0.96 |
The theoretical s20, w values were predicted using HYDROPRO. The frictional ratios from the SEDFIT analyses ranged between 1.2 for the shorter collagen peptides up to 1.5 for the longer ones.
n.a. Not available.