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. 2022 Dec 31;299(2):102857. doi: 10.1016/j.jbc.2022.102857

Figure 1.

Figure 1

SOD1 forms misfolding by oxidative stress in cells and in vitro.A, thio T fluorescence assay of 40 μM apo-SOD1 with or without 200 μM H2O2. The intensity was normalized with the highest intensity as 100% and the lowest intensity as 0%. n = 3 biologically independent samples, data are presented as mean values ± S.D. B, TEM image of 40 μM, oxidized apo-SOD1. Scale bars represent 500 nm. C, confocal microscopy images of EGFP-SOD1 aggregates (arrows) in both N2a and HEK-293T cells after H2O2 treatment (100 μM, 3 h). Scale bar represents 25 μm, 10 μm. D, FRAP of the condensates formed by oxidized SOD1 in HEK-293T cells. The intensity was normalized with the pre-bleached as 100% and the first time point after bleaching as 0%. n = 3 biologically independent samples, data are presented as mean values ± S.D. Scale bar represents 10 μm. FRAP, fluorescence recovery after photobleaching; SOD, superoxide dismutase; TEM, transmission electron microscope.