REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
APC/Cyanine7 anti-mouse CD326 (Ep-CAM) (1:100)	BioLegend	Clone G8.8, Cat# 118218; RRID: AB_2098648
BV605 Rat Anti-mouse CD45 (1:100)	BD Biosciences	Clone 30-F11, Cat# 563053; RRID: AB_2737976
V450 Hamster Anti-mouse TCR- β chain (1:100)	BD Biosciences	Clone H57-597, Cat# 560706; RRID: AB_1727576
PerCP-CyTM5.5 Rat Anti-CD11b (1:100)	BD Biosciences	Clone M1/70, Cat# 550993; RRID: AB_394002
PE-CyTM7 Rat Anti-Mouse Ly-6G (1:100)	BD Biosciences	Clone 1A8, Cat# 560601; RRID: AB_1727562
Alexa Fluor® 700 Rat Anti-Mouse Ly-6C (1:100)	BD Biosciences	Clone AL-21, Cat# 561237; RRID: AB_10612017
PE-CF594 Rat Anti-Mouse CD31 (1:100)	BD Biosciences	Clone MEC 13.3, Cat# 563616; RRID: AB_2738320
Rat anti-mouse CD16/32 (Fc block) (1:100)	BD Biosciences	Clone 2.4G2, Cat# 553142; RRID: AB_394656
Bacterial and virus strains
Herpes simplex virus-1 (McKrae) strain	Rana et al.1	Available upon request
Chemicals, peptides, and recombinant proteins
DMEM/F-12	Thermo Fisher Scientific	Cat# 11320033
Dispase® II (neutral protease, grade II)	MilliporeSigma	Cat# 04942078001
HEPES Buffered Saline Solution	MilliporeSigma	Cat# C-40020
D-sorbitol	MilliporeSigma	Cat# S3889
Antibiotic/ Antimycotic	Thermo Fisher Scientific	Cat# 15240062
PBS, pH 7.2	Thermo Fisher Scientific	Cat# 20012050
Trypsin-EDTA (0.25%), phenol red	Thermo Fisher Scientific	Cat# 25200056
Trypsin Inhibitor from soybean	MilliporeSigma	Cat# C10109886001
DPBS, calcium, magnesium	Thermo Fisher Scientific	Cat# 14040133
Liberase™ TL Research Grade	Sigma-Aldrich	Cat# 5401020001
RPMI 1640 medium, HEPES	Thermo Fisher Scientific	Cat# 2240001
Fetal Bovine Serum	Thermo Fisher Scientific	Cat# A47688-01
L-Glutamine solution, 200mM	Sigma-Aldrich	Cat# G7513
Penicillin-Streptomycin	Sigma-Aldrich	Cat# P0781
Sodium Pyruvate solution, 100mM	Sigma-Aldrich	Cat# S8636
Sodium Bicarbonate (Powder/Certified ACS)	Fisher scientific	Cat# S233
2-Mercaptoethanol	Sigma-Aldrich	Cat# M3148
Ketathesia-Ketamine hydrochloride injection	Henry Schein Animal Health	NDC code: 11695-0701-1
Xylazine Sterile Solution (20 mg/mL)	Animal Health International	Cat# 20101547 Mfr# 59399-0110-20
Critical commercial assays
LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit for 405 nm excitation	Invitrogen	Cat# L34966
UltraComp eBeads™ Compensation Beads	Invitrogen	Cat# 01-2222-42
Experimental models: Organisms/strains
Mouse: C57BL/6J (female), 8–12 weeks old	The Jackson Laboratory	Stock# 000664
Software and algorithms
FlowJo Version v10.4	Tree Star	https://www.flowjo.com
BD FACSDiva Software	BD Biosciences	https://www.bdbiosciences.com/enus/products/software/instrument-software/bd-facsdiva-software
Other
50 mL tubes	Corning	Cat# 352098
15 mL tubes	Corning	Cat# 352099
1.7 mL tubes	Thomas Scientific	Cat# 1149K01
1.5 mL tubes	Fisher Scientific	Cat# 05-408-129
0.22 μm syringe filters	Fisher Scientific	Cat# 09-720-004
3 mL syringes	Fisher Scientific	Cat# 14-823-435
10 mL syringes	Fisher Scientific	Cat# 14-823-2A
24-well plates	Corning	Ref# 3524
96-well U-bottom FACS plates	Corning	Ref# 353077
Medium Petri dish	Corning	Ref# 351007
27G (1/2 in.) needle	BD	Cat# 305109
Cell strainers 70 μm	Fisher scientific	Cat# 22363548
Curved forceps	Integra Miltex	Cat# 18-782
Straight forceps	Integra Miltex	Cat# 18-780
Castroviejo scissors	Integra Miltex	Cat# 18-1576
Eppendorf 5424/5424R centrifuge	Eppendorf	Rotor Cat# FA-45-24-11
Eppendorf 5810R centrifuge	Eppendorf	Rotor Cat# S-4-104
Inverted microscope	Leica DMi1	N/A
Stereo microscope	Leica S8APO	N/A
Slit lamp microscope	Kowa SL-15	N/A
Orbital shaker	Fisher scientific	N/A
Disruptor Genie	Scientific Industries	N/A
Incubator (Heratherm™)	Thermo Fisher Scientific	N/A
CO2 incubator (Heracell™ 150i)	Thermo Fisher Scientific	N/A
Class II Biosafety Cabinet	Labconco	N/A
BD LSRFortessa flow cytometer	BD Biosciences	N/A