Skip to main content
. 2023 Jan 25;4(1):101545. doi: 10.1016/j.xpro.2022.101545

YPD liquid medium

Reagent Final concentration Amount
yeast extract 1% (w/v) 10 g
Peptone 2% (w/v) 20 g
ddH2O n/a Adjust to 950 mL
Glucose 40%, sterile, add last and after autoclaving 2% 50 mL
Total n/a 1 L

Autoclave, store at room temperature (20°C–25°C), add glucose just before usage, store unused medium at 4°C.

Can be stored for a year.