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10× TE buffer pH 7.4, for yeast transformation
| Reagent | Final concentration | Amount |
|---|---|---|
| TRIS | 100 mM | 1.211 g |
| EDTA disodium salt dihydrate | 10 mM | 372 mg |
| HCl Hazard: This is an acid, wear gloves and handle solution in chemical hood |
Adjust pH to 7.4 | As required |
| ddH2O | n/a | To final of 100 mL |
| Total | n/a | 100 mL |
Autoclave, store at room temperature (20°C–25°C), can be stored for several years.
