Sample	A	B	C	D
	Double digest	Single digest controls		No digest control
Plasmid	5 μg	0.5 μg	0.5 μg	0.5 μg
10× reaction buffer, compatible for both restriction enzymes	20 μL	2 μL	2 μL	2 μL
Restriction enzyme 1	2 μL	0.2 μL	n/a	n/a
Restriction enzyme 2	2 μL	n/a	0.2 μL	n/a
ddH2O, to final volume of	200 μL	20 μL	20 μL	20 μL