Sample number	Herring sperm DNA	Circular plasmid	Linearized plasmid optimal dilution, as determined in step 3	PCR-3 amplicon∗
1	5 μL	1 μL	n/a	n/a
2	5 μL	n/a	5 μL	n/a
3	5 μL	n/a	n/a	5 μL PCR-3 amplicon #1
4	5 μL	n/a	5 μL	5 μL PCR-3 amplicon #1
5	5 μL	n/a	n/a	5 μL PCR-3 amplicon #2
6	5 μL	n/a	5 μL	5 μL PCR-3 amplicon #2
. . .				etc
x-2	5 μL	n/a	n/a	5 μL PCR-3 amplicon #n
x-1	5 μL	n/a	5 μL	5 μL PCR-3 amplicon #n
Final tube x	5 μL	n/a	n/a	n/a

∗ The amplicons were generated in step 1. Each amplicon (numbered from #1 to #n) represents an amplicon with a specific mutation, as well as the un-mutated amplicon if required.