Solution I (0.1 M Lithium Acetate solution):
| Reagent | Final concentration | Amount |
|---|---|---|
| ddH2O | n/a | 160 mL |
| 10× TE buffer (pH 7.4), sterile | 1× | 20 mL |
| 1 M lithium acetate, sterile | 100 mM | 20 mL |
| Total | n/a | 200 mL |
Autoclave 160 mL of double deionized H2O (ddH2O) in a bottle, then add the other components, aseptically.
Store at room temperature (20°C–25°C), can be stored for several years.