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. 2023 Jan 25;4(1):101545. doi: 10.1016/j.xpro.2022.101545

Solution I (0.1 M Lithium Acetate solution):

Reagent Final concentration Amount
ddH2O n/a 160 mL
10× TE buffer (pH 7.4), sterile 20 mL
1 M lithium acetate, sterile 100 mM 20 mL
Total n/a 200 mL

Autoclave 160 mL of double deionized H2O (ddH2O) in a bottle, then add the other components, aseptically.

Store at room temperature (20°C–25°C), can be stored for several years.