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. 2023 Jan 25;4(1):101545. doi: 10.1016/j.xpro.2022.101545

YPD solid medium

Reagent Final concentration Amount
yeast extract 1% (w/v) 10 g
Peptone 2% (w/v) 20 g
Agar 2% (w/v) 20 g
ddH2O n/a Adjust to 950 mL
Glucose 40%, sterile, add last and after autoclaving 2% 50 mL
Total n/a 1 L

Autoclave, let cool to 40°C–50°C (can hold bottle with bare hands without burning the skin), add glucose, pour into petri plates, let solidify, store at room temperature (20°C–25°C) for 2 days, then seal in a bag and store at 4C.

Can be stored for a year.