YPD solid medium
| Reagent | Final concentration | Amount |
|---|---|---|
| yeast extract | 1% (w/v) | 10 g |
| Peptone | 2% (w/v) | 20 g |
| Agar | 2% (w/v) | 20 g |
| ddH2O | n/a | Adjust to 950 mL |
| Glucose 40%, sterile, add last and after autoclaving | 2% | 50 mL |
| Total | n/a | 1 L |
Autoclave, let cool to 40°C–50°C (can hold bottle with bare hands without burning the skin), add glucose, pour into petri plates, let solidify, store at room temperature (20°C–25°C) for 2 days, then seal in a bag and store at 4C.
Can be stored for a year.