Tyrode solution
| Reagent | Final concentration | Amount |
|---|---|---|
| NaCl | 140 mM | 8.18 g |
| KCl | 5 mM | 0.37 g |
| CaCl2 | 2.5 mM | 0.37 g |
| MgCl2 | 2 mM | 0.4 g |
| HEPES (1 M) | 10 mM | 10 mL |
| Total | N/A | 1 L |
Store at 4°C for six months.
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