REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
Hyaluronidase	Sigma-Aldrich	Cat# H4272
29-gauge needle	Exel International	Cat# EU12913
NaCl	Sigma-Aldrich	Cat# S9888
KCl	Sigma-Aldrich	Cat# 529552
CaCl2	Sigma-Aldrich	Cat# 21115
MgCl2	Sigma-Aldrich	Cat# M8266
HEPES	Sigma-Aldrich	Cat# H3375
Type I collagenase	Sigma-Aldrich	Cat# C5138
Glass-bottomed dishes	Nest Scientific	Cat# 801001
mCherry-EGFP-LC3	pEGFP-LC3 (Addgene 24920) was double digested with AfeI (blunt) and BamHI, yielding the 1.2 kb EGFP-LC3 segment. This segment was subcloned into pmCherry-C1 (Addgene 632524), which was opened by the digestion of SmaI (blunt) and BamHI.
Experimental models: Organisms/strains
C57Bl/6 mice, male, 17 weeks	The Jackson Laboratory	Cat# 000664
Software and algorithms
Fiji ImageJ v1.53f	Schindelin et al.	https://imagej.net/software/fiji/
Macro: FFTmuscle.ijm	This paper	https://github.com/novashining/FFTmuscle
Example image folder – contains sample images to run the Macro	This paper
Other
Electroporation	Harvard Apparatus	ECM 830
Confocal Microscope	Zeiss	LSM 780