Table 3.

T-ARMS-PCR Primer Sequences, Melting Temperatures (T_m), and the Size of PCR Product for Every Genotype

SNP	Primer Sequence	PCR Product	Genotype (Blood Pressure)
rs2956540	Forward inner primer (C allele): ACTTATTTTTCCTCCATTTGCTAAGCC	209	CC (417, 209)
	Reverse inner primer (G allele): GTTTTATGCTGAAAATAGAATAGTGGTAGC	265	GG (417, 265)
	Forward outer primer (5' - 3'): CTGACATAGATTTTAACTGACACGCATT	417	CG (417, 265, 209)
	Reverse outer primer (5' - 3'): CAGTCCACAATGAAGAACAAAAATTTAC
rs10519694	Forward inner primer (C allele): AAATATTCACATCAATAAGTAAATGAAGGC	252	CC (402, 252)
	Reverse inner primer (T allele): TATTTTTCTCCTCCCAGCCTGTAGACGA	208	TT (402, 208)
	Forward outer primer (5' - 3'): TGGTTTTGAGTTTAGGTAATCAAGGTCC	402	CT (402, 252, 208)
	Reverse outer primer (5' - 3'): TGCTAGAATTGAATGGCAGTATTGAGTT

SNP = single nucleotide polymorphism; PCR = polymerase chain reaction; T-ARMS = tetra-primer amplification refractory mutation system.