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. 2023 Feb 4;8:9. doi: 10.1038/s41541-023-00604-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Functional impairment of the heart before (•) and after injection of antigen (▪) was assessed by ECG (a). Statistical analysis performed by two-way ANOVA. Significance to before injection shown, *P < 0.05, **P < 0.01, ***P < 0.001. Development of carditis (b). Inflammatory changes in the myocardium and valvular tissue, characterised by mononuclear cell infiltration, were scored in PBS (•), rM5 (▪), P*17-DT (▴) and K4S2-DT (♦) treated rats. Statistical analysis performed by one-way ANOVA. Significance to PBS group shown, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Error bars represent standard deviation. Histological changes in cardiac tissues following exposure to PBS, P*17-DT, K4S2-DT and rM5 (c). Arrows indicates the possible mononuclear cell infiltration. Representative images from each group shown, scale bar represents 50 µm.