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. 2023 Feb 4;8:9. doi: 10.1038/s41541-023-00604-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Antigen-specific serum IgG titres (a) and serum IgG reactivity in rats administered with PBS (•), rM5 (▪), P*17-DT (▴) or K4S2-DT (♦) to peptide p*17 (b), peptide K4S2 (c) and rM5 (d). Absorbance values of day-35 rat sera at 1:400 (b, c) and 1:100 (d) is shown. IgG titres were defined as the highest dilution of serum for which OD was >3 standard deviations above the mean OD of PBS control at highest dilution. Statistical analysis performed by one-way ANOVA with Dunnett multiple comparison test. Significance to PBS control group, *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001. Error bars represent standard deviation.