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. 2023 Feb 4;8:9. doi: 10.1038/s41541-023-00604-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Detection of pro-inflammatory IL-17A in the sera and cardiac tissues of rats injected with PBS, rM5, P*17-DT or K4S2-DT. IL-17A in sera was assessed by ELISA (a). Absorbance values at day-35 are shown. Statistical analysis performed by one-way ANOVA with multiple comparison test. Significance to PBS control group, *P < 0.05 and ****P < 0.0001. Error bars represent standard deviation. Tissue-specific localisation of IL-17A in the myocardium and valvular tissues of rats was investigated by immunohistochemical staining (b). Representative images from two rats in each group given (b, S1). Images magnified at ×200, snippets magnified at ×400, and scale bar represents 50 µm.