Figure 3.

Targeting of optogenetic inactivation and neural responses. A, Imaging viral expression in ferret auditory cortex. Top, Widefield imaging of coronal sections through the ectosylvian gyrus with the cell bodies labeled with DAPI (blue) and ChR2 labeled with mCherry (red). Middle/Bottom, Confocal imaging of the injection site showing colocalization of cell bodies and mCherry expression (outlined). B, Experimental schematic showing stimulus and light delivery protocols. C, Configurations of probe and optic fiber over injection sites within MEG in each ferret (F1807 and F1801). D, Peristimulus time histogram and raster plots showing responses of four example units recorded from auditory cortex with and without light delivery in a single laser pulse (columns 1-3) and a 10 Hz laser pulse (column 4).