Figure 3.

Delayed injection of lumateperone after LPS (30 min later) confirms anti-inflammatory activity in hippocampal tissue. A, Adult mice first received a subcutaneous injection of either LPS (500 µg/kg diluted in 0.9% saline) or saline. Thirty minutes later, mice were injected intraperitoneally with either lumateperone (3 mg/kg) or its vehicle (5% DMSO, 5% Tween 20, 15% PEG 400, and 75% water). B, Results show hippocampal mRNA levels of Il1b, Tnfa, Il6, Il10, and (C) Icam1, Cldn5, and Csf1 normalized to the control group using the Dct method. Graphs are mean ± SEM; n = 4–9 per group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, compared with control group (two-way ANOVA followed by Tukey's multiple comparison test). The control group received vehicle+saline injections. Dct, δ ct; Luma, lumateperone; Tnfa, tumor necrosis factor-α.