Figure 4.

Lumateperone normalized markers of inflammation that were altered after 2 h of acute restraint stress. Adult mice were injected intraperitoneally with either lumateperone (3 mg/kg) or its vehicle (5% DMSO, 5% Tween 20, 15% PEG 400, and 75% water). For the groups receiving restraint stress, after treatment, mice were immediately placed in a restraint bag for a duration of 2 h while the control group went back into their cages. A, Experimental design. B, Results show blood serum protein levels of IL-1β, TNF-α, IL-6, and IL-10 normalized to control group, using Multiplex MSD V-plex technology where results are expressed in pg/ml. C, Corresponding hippocampal levels of Il1b, Tnfa, Il6, and Il10 mRNA normalized to control group (nonstressed, vehicle) using the Dct method. D, Corticosterone was measured in serum with an ELISA. E, Cldn5 hippocampal mRNA levels. Graphs are mean ± SEM; n = 11–13 per group. *p < 0.05, **p < 0.01, ***p < 0.001, compared with control group (vehicle, nonstressed) using one-way ANOVA followed by Bonferroni multiple comparison test. Dct, δ ct; Luma, lumateperone; TNF-a, tumor necrosis factor alpha.