Table 1.
Western blot antibodies references and concentrations
| kDa | Antibody name | Source | Brand | Reference | Dilution |
|---|---|---|---|---|---|
| 289 | Phospho-mTOR (Ser2448) | Rabbit | Cell Signaling | 5536 | 1:1000 |
| 289 | mTOR | Mouse | Cell Signaling | 4517 | 1:1500 |
| 70-85 | Phospho-p70 S6 kinase (Thr389) | Mouse | Cell Signaling | 9206 | 1:600 |
| 70-85 | p70 S6 kinase | Rabbit | Cell Signaling | 2708 | 1:1000 |
| 60 | Phospho-Akt (Thr308) | Rabbit | Cell Signaling | 4056 | 1:400 |
| 60 | Akt (pan) | Mouse | Cell Signaling | 2920 | 1:700 |
| 45 | β-actin | Mouse | Cell Signaling | 3700 | 1:6000 |
| 44-42 | Phospho-p44/42 MAPK (Erk1/2) | Rabbit | Cell Signaling | 4370 | 1:1500 |
| 44-42 | p44/42 MAPK (Erk1/2) | Mouse | Cell Signaling | 4696 | 1:1500 |
MAPK, mitogen-activated protein kinase; mTOR, mammalian target of rapamycin.