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. 2023 Feb 1;43(5):736–748. doi: 10.1523/JNEUROSCI.1504-22.2022

Figure 5.

Figure 5.

Estrous cycle stage during AVSS influences time spent grooming in the the splash test. a, Timeline. b, AVSS does not significantly alter time spent grooming (t(41) = 1.9, p = 0.06, n =21–22). c, AVSS increases the latency to groom (t(41) = 3.1, p = 0.003, n = 21–22). d, Estrous phase has no significant effect on time spent grooming during the splash test in nonstressed mice (open circle) (F(3,17) = 2.04, p = 0.15, n = 5–6 per cycle phase). AVSS during the estrus phase decreases the time spent grooming (black circles) (2-way ANOVA, cycle × stress interaction, F(3,35) = 4.20, p = 0.01, n = 5–6 mice per cycle phase). e, There is no significant interaction between cycle and stress in latency to groom (2-way ANOVA, cycle × stress interaction, F(3,35) = 1.65, p = 0.20, n = 5–6 mice per cycle phase). (*p < 0.05, **p < 0.01) Error bars indicate mean ± SEM.