TABLE 7.

Sites of hydrolytic cleavage of Nod factors of Rhizobium meliloti by enzymes isolated from the roots of Medicago sativa^a  

Hydrolase (reference)	No. of N-acetylglucosamine residues	R1	R2	Cleavage site(s)
Intact roots (258, 260)	IV	Sulfatyl	H	a
	IV	H	H	a, b
	IV	Sulfatyl	O-Acetyl	a
	V	Sulfatyl	H	c, d
	V	H	H	c, d, e
Chitinase class I (241, 258)	IV	Sulfatyl	H	None
	IV	H	H	b
	IV	Sulfatyl	O-Acetyl	None
	V	Sulfatyl	H	d
	V	H	H	d, e
Novel chitinase/lysozyme (179)	IV	Sulfatyl	H	b
	IV	H	H	b
	IV	Sulfatyl	O-Acetyl	b
	V	Sulfatyl	H	d
Nod factor hydrolase (258, 260)	IV	Sulfatyl	H	a
	IV	H	H	a
	IV	Sulfatyl	O-Acetyl	a
	V	Sulfatyl	H	c
	V	H	H	c
	V	Sulfatyl	O-Acetyl	c

^aDistinct cleavage site preferences exist toward tetrameric (IV) and pentameric (V) Nod factors of R. meliloti as well as their desulfated and deacetylated derivatives, respectively.