Fig. 5.

PirB activates the RhoA/ROCK2/LIMK1/cofilin signalling pathway in CSR mice. a Western blot analysis of hippocampal RhoA, ROCK2, pLIMK1/LIMK1, and pcofilin/cofilin levels in control, CSR, PirB cKO, and PirB cKO + CSR mice. b–e Ratio of fold change from Western blot. The RhoA (n = 5) and ROCK2 (n = 5) levels were significantly higher in CSR mice than in control and PirB cKO + CSR mice. The phosphorylated LIMK1 (n = 5) and cofilin (n = 5) levels were significantly lower in CSR mice than in control and PirB cKO + CSR mice. f Western blot analysis of hippocampal ROCK2, pLIMK1/LIMK1, and pcofilin/cofilin levels in control and CSR mice after fasudil treatment. g–i Ratio of fold change from Western blot. The ROCK2 level was significantly lower in fasudil + CSR mice than in CSR mice (n = 5). The phosphorylated LIMK1 (n = 5) and cofilin (n = 5) levels were significantly higher in fasudil + CSR mice than in CSR mice. Data are presented as the means ± SD and were analysed by one-way ANOVA with Tukey’s test (*p < 0.05, **p < 0.01)